南昌近郊辣椒两种炭疽病菌的初步研究

1962年秋,作者等在南昌近郊,从感病长果种辣椒的果上,分离到两种炭疽病菌。一种分生孢子为椭圓形(以下簡称G菌),病斑圆形或椭圓形,凹陷,灰綠色或黄色,以后变棕色。另一种分生孢子为鐮刀形(以下簡称V菌),病斑不定形,暗棕色至黑色。这两种病菌,在馬鈴薯、洋菜培养基上都能形成孢子,且对辣椒都有寄生性。     

颗粒剂防治玉米钻心虫的研究

1962年在北京夏玉米上用顆粒剂防治玉米钻心虫的結果表明:心叶末期施5％DDT顆粒剂,防治一次,每株2克,在一般年份,从产量上来看是很有利的。同时对药剂残效,幼虫在玉米不同生育期植株上的分布,以及顆粒剂在心叶期施药至抽雄后在植株上的分布测定,并結合顆粒剂小区防治試驗。分析結果说明:心叶末期施药,影响药效的主要原因,不是5％DDT残效的問題,而是抽雄后药剂在植株上部分布較少,尤其是包在雄穗节外的第一叶腋没有药,而此时期,幼虫在植株上部分布較多,同时DDT速效也不如666。     

Altered adenosine triphosphatase activities in pigs with naturally occurring hypertrophic cardiomyopathy

The purpose of this study was to determine whether myocardial adenosine triphosphatase (ATPase) activities were reduced in pigs with naturally occurring hypertrophic cardiomyopathy (HCM). The selection of hearts for the HCM and the normal control groups depended on histological examination. Specific ATPase activity and 5-nucleotidase activity were measured in left ventricular myocardium obtained from HCM (n=7) and normal control (n=7) animals. The histological features of HCM included marked disorientation of muscle cells, thickening of the intramural coronary arterial wall with a narrowed lumen, endocardial fibrosis and myocardial fibrosis. The HCM group showed significant increases in both heart weight (32%) and heart weight to body weight ratio (46%). The total ATPase activity in crude homogenates from the HCM group was significantly decreased by 16%. Azide-sensitive ATPase (mitochondrial ATPase) activity, ouabain-sensitive ATPase (Na⁺,K⁺-ATPase) activity, basal Mg²⁺-ATPase activity and Ca²⁺-ATPase activity were all significantly decreased by 18%, 30%, 20% and 50%, respectively. In contrast, no significant decrease was found in the mean values for 5-nucleotidase activity. These results suggest that myocardial ATPase activities are suppressed in pigs with naturally occurring HCMAbbreviations ATP adenosine triphosphate - gww grams wet weight - HCM hypertrophic cardiomyopathy - Pi inorganic phosphate     

The in vivo sensitivity of ATPases to DDT,DDE, and physical immobilization in American cockroaches

American cockroaches injected with sublethal doses of DDT (0.75 μg/roach) at 5-day intervals showed a 40% reduction in oligomycin-sensitive Mg²⁺ATPase from muscle homogenates, and a 23% reduction of Na⁺-K⁺ATPase from nerve cords. Thus, the maximum effect measured occurred with the same enzyme and tissue as determined from in vitro studies. The metabolite, DDE, used at 15 μg per roach, gave no significant change in activity of the ATPase system following injection. In contrast, high single doses of DDT (7.5 μg/roach) and 100 μg DDE and dicofol per roach caused over 30% increase in oligomycin-sensitive Mg²⁺ATPase of muscle and a 10–15% increase in Na⁺-K⁺ATPase of nerve cords measured 24 and 48 hr later. While a similar response was observed for Mg²⁺ATPase activities in cockroaches that were immobilized, the increase in enzyme activities were much greater than that caused by the pesticides.     

草莓杯装软罐头的研制

试验对杯装草莓罐头的工艺进行了研究。结果表明,烫漂的工艺为75 ℃、4 min(分钟);注液汤汁的配比为白砂糖25 %、柠檬酸0.3 %、氯化钙0.2 %;杀菌工艺为7.5 kGy辐射杀菌。     

苹果采前落果与内源激素的关系

为了探讨苹果采前落果与内源激素之间的关系,在采前8周间定期测定了不同苹果品种的果柄、果台和离层形成部位组织中IAA、ABA含量;在采收前20d内定期测定了离层部位组织中细胞壁分解酶(Cellulase)的活性;在收获期测定了果实乙烯的发生量。结果表明:不同品种果柄、果台和离层部位组织中IAA和ABA含量变化有差异,但它们变化的总趋势相似,都是随着果实成熟IAA含量下降,而ABA含量上升;不同品种的成熟果实中乙烯发生量有很大差异,以落果多的品种显著大于落果少的品种;采前落果重的品种离层部位组织中细胞壁分解酶的活性在果实成熟期急剧增加。由于果实进入成熟阶段后,IAA含量下降,ABA含量升高,ABA/IAA之间的相对平衡被打破,高ABA/IAA以及高乙烯会刺激离层组织中细胞壁分解酶的活性增高,进而促进离层形成,这可能是导致落果发生的原因。     

不同含水量菜豆种子老化过程中生理特性的研究

 对不同含水量的菜豆种子( 2.1%、3.4%、3.8%、6.9%和9.0% ) 老化过程中的生理特性进行了研究。结果表明, 菜豆种子在含水量为318%时, 发芽率, 保护酶系统( SOD、POD和CAT) 活性和脱氢酶活性明显高于其它含水量的种子, 而脂氧合酶(LOX) 活性和丙二醛(MDA) 含量显著低于其它含水量的种子, 表现出较高的生活力。此结果表明适度降低菜豆种子的含水量可以提高其抗老化劣变能力;不同生活力的菜豆种子POD同工酶酶谱表现不同, 而SDS2PAGE蛋白质谱带则基本相同。     

难忘的起步阶段

时光似箭,日月如梭。转眼,中国饲料工业协会成立20周年了,中国饲料工业也走过了从起步到腾飞的光辉历程。1985年我开始接触这个新兴行业直到1995年退休。作为一名具体工作的行业管理工作者,我度过了从艰难创业到蓬勃发展的不平凡的十年。这其中有丰收的喜悦,也有创业的艰辛;有成功的快乐,也有阻力和冷漠带来的苦涩。回忆起步阶段的十年,酸甜苦辣,百感交集。从创业到蓬勃发展1985年,山东省政府决定全省饲料工业的统筹管理由省计委移交省经委后,我开始踏入这个新的战斗行列。省政府对饲料工业是重视的。早在1985年前就成立了全省饲料工业领导…     

Comparison of morphology, viability, and function between blood and milk neutrophils from peak lactating goats

The morphological features of blood and milk neutrophils from peak lactating goats were compared using light microscopy, scanning electron microscopy and flow cytometry in order to investigate the cytological changes of neutrophils after migration into the mammary gland. The kinetics of reactive oxygen intermediates (ROI) generation and gelatinase release of blood and milk neutrophils, with or without stimulation of phorbol 12-myristate, 13-acetate ester (PMA), were used to characterize their responses to inflammatory stimuli. Neutrophils isolated from goat milk were highly segmented and contained multi-lobed nuclei. Ultrastructurally, milk neutrophils were more ruffled on the surface compared to blood neutrophils. Approximately 30% of milk neutrophils were undergoing cell death, either necrosis or apoptosis, in contrast to 8% of blood neutrophils. The ROI production of activated milk neutrophils peaked earlier than blood neutrophils, but the duration and the intensity were much less. Neutrophils from both sources augmented the release of gelatinase in response to PMA (1 ng/mL). However, the amount of gelatinase released from milk neutrophils was lower (P < 0.05) than that of blood neutrophils. In summary, more neutrophils become apoptotic and necrotic in the mammary gland, presumably due to spontaneous aging, the process of diapedesis, and the interaction with milk components. Milk neutrophils have impaired functionalities in comparison with blood neutrophils. The information is relevant when studying mammary gland immunity and related diseases, such as mastitis.